BD Pharmingen 7-AAD核酸染料 559925
2025-07-18
![]() |
BD Pharmingen 7-AAD核酸染料 559925,7-Amino-Actinomycin D (7-AAD)
(7-AAD) 是一種即用型核酸染料,可替代碘化丙啶(PI)用于流式細(xì)胞檢測(cè)中非活細(xì)胞的排除。與PI相比,7-AAD的優(yōu)勢(shì)在于能與藻紅蛋白(PE)和異硫氰酸熒光素(FITC)標(biāo)記的單克隆抗體聯(lián)用,進(jìn)行雙色分析,且7-AAD、PE和FITC的熒光發(fā)射光譜重疊極小。7-AAD的熒光在遠(yuǎn)紅光光譜范圍內(nèi)檢測(cè)(650 nm長(zhǎng)通濾光片)。
該試劑作為一種活力探針,基于光散射和FL3通道檢測(cè)的7-AAD攝取,用于死細(xì)胞排除方法。無(wú)需稀釋,建議用量為每測(cè)試(1×10^6個(gè)細(xì)胞)添加5 µl(0.25 µg),分析前孵育10分鐘。
7-Amino-Actinomycin D (7-AAD) is a convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays. The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labelled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter). This reagent is used as a viability probe for methods of dead cell exclusion, based on light scatter and uptake of 7-AAD as detected in FL3. This reagent does not require dilution. Suggested quantity to use: 5 µl (0.25 µg)/test (1x10^6 cells) and incubate for 10 minutes before analysis.
BD Pharmingen 7-AAD核酸染料 559925,7-Amino-Actinomycin D (7-AAD)
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
江蘇省科學(xué)器材有限公司
- BD CBA人Th1/Th2細(xì)胞因子試劑盒II 551
- BD 人間充質(zhì)干細(xì)胞分析試劑盒 562245
- BD CBA人炎性細(xì)胞因子試劑盒I 551811
- BD CBA Human Chemokine Kit 552990
- BD GolgiPlug蛋白轉(zhuǎn)運(yùn)抑制劑(含Brefeld
- BD Perm/Wash 洗滌緩沖液 554723
- BD Phosflow Perm 緩沖液 III 558050
- BD Pharm Lyse 裂解緩沖液555899
- BD Pharmingen FITC小鼠抗人CD3 555339
- BD 人多能干細(xì)胞分選和分析試劑盒 5604
- BD 人定向胰腺內(nèi)胚層分析試劑盒562496
- BD Pharmingen FITC小鼠抗人CD90 5555