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BD Pharmingen 7-AAD核酸染料 559925

2025-07-18

產(chǎn)      地:
美國(guó)
所在地區(qū):
江蘇南京市
有效期還剩 84舉報(bào)該信息

BD Pharmingen 7-AAD核酸染料 559925,7-Amino-Actinomycin D (7-AAD)


(7-AAD) 是一種即用型核酸染料,可替代碘化丙啶(PI)用于流式細(xì)胞檢測(cè)中非活細(xì)胞的排除。與PI相比,7-AAD的優(yōu)勢(shì)在于能與藻紅蛋白(PE)和異硫氰酸熒光素(FITC)標(biāo)記的單克隆抗體聯(lián)用,進(jìn)行雙色分析,且7-AAD、PE和FITC的熒光發(fā)射光譜重疊極小。7-AAD的熒光在遠(yuǎn)紅光光譜范圍內(nèi)檢測(cè)(650 nm長(zhǎng)通濾光片)。

該試劑作為一種活力探針,基于光散射和FL3通道檢測(cè)的7-AAD攝取,用于死細(xì)胞排除方法。無(wú)需稀釋,建議用量為每測(cè)試(1×10^6個(gè)細(xì)胞)添加5 µl(0.25 µg),分析前孵育10分鐘。

7-Amino-Actinomycin D (7-AAD) is a convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays. The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labelled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).  This reagent is used as a viability probe for methods of dead cell exclusion, based on light scatter and uptake of 7-AAD as detected in FL3.  This reagent does not require dilution.  Suggested quantity to use:  5 µl (0.25 µg)/test (1x10^6 cells) and incubate for 10 minutes before analysis.



BD Pharmingen 7-AAD核酸染料 559925,7-Amino-Actinomycin D (7-AAD)


Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.


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